RhoA ELISA Kit BK150 from Cytoskeleton, Inc.

Product Specs
ItemRhoA ELISA Kit
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberBK150
Detection TargetRhoA
QuantityKIT 96 assays
MethodRho G-LISA Assay Kits
ApplicationsELISA
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndromeJournal of translational medicineJune 18, 2023Zhai R, Lenga Ma Bonda W, Leclaire C, Saint-Béat C, Theilliere C, Belville C, Coupet R, Blondonnet R, Bouvier D, Blanchon L, et al.results were normalized to the total RhoA level as measured using the Total RhoA ELISA Biochem Kit (BK150, Cytoskeleton, Denver, USA). Active RhoA was determined in duplicate with the same colorimetric RhoA

(2) Dual mechanism underlying failure of neural tube closure in the Zic2 mutant mouseDisease Models & MechanismsMarch 1, 2023Sarah Escuin, Saba Rose Rose Raza-Knight, Dawn Savery, Carles Gaston-Massuet, Gabriel L. Galea, Nicholas D. E. Greene, Andrew J. Coppinstructions (Escuin et al., 2015). Total RhoA was determined by enzyme-linked immunosorbent assay (ELISA; BK150, Cytoskeleton Inc.). Assays were performed in duplicate on three biological replicates for each genotype

(3) AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defenceNatureSeptember 1, 2021Lee S, Karki R, Wang Y, Nguyen LN, Kalathur RC, Kanneganti TD.Cytoskeleton) and normalized to total RhoA levels, which were measured using the Total RhoA ELISA kit (BK150, Cytoskeleton) according to the manufacturer’s instructions. For pull-down assays, a GST-tagged Rho

(4) DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular CalcificationArteriosclerosis, Thrombosis, and Vascular BiologyJuly 1, 2020David Ngai, Marsel Lino, Katheryn E. Rothenberg, Craig A. Simmons, Rodrigo Fernandez-Gonzalez, Michelle P. BendeckCytoskeleton) and normalized to total RhoA measured by RhoA E-LISA (enzyme-linked immunosorbent assay; BK150; Cytoskeleton) according to the manufacturer’s instructions. Immunocytochemistry To assess stiffness-dependent

(5) Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathwayNature materialsJune 1, 2020Chronopoulos A, Thorpe SD, Cortes E, Lachowski D, Rice AJ, Mykuliak VV, Róg T, Lee DA, Hytönen VP, Del Río Hernández AE.RhoA-GTP were quantified using total RhoA ELISA and active RhoA G-LISA assay kits (Cytoskeleton, Inc. BK150 and BK124) according to the manufacturer’s instructions. Immediately after treatment, cells were rinsed

(6) Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neuronsPLoS biologySeptember 1, 2019Haq N, Schmidt-Hieber C, Sialana FJ, Ciani L, Heller JP, Stewart M, Bentley L, Wells S, Rodenburg RJ, Nolan PM, et al.(Cytoskeleton, BK128). The activity of RhoA was normalised to the total level of RhoA (Cytoskeleton, BK150). Immunofluorescence and image acquisition Primary hippocampal cultures were prepared from E16.5 C57BL/6Ntac

(7) Nano-topography: Quicksand for cell cycle progression?Nanomedicine: Nanotechnology, Biology and MedicineNovember 1, 2018Marianna Giannini, Chiara Primerano, Liron Berger, Martina Giannaccini, Zhigang Wang, Elena Landi, Alfred Cuschieri, Luciana Dente, Giovanni Signore, Vittoria Raffaextracted and chilled in N2. After normalization of protein concentration, quantification of total RhoA (BK150, Cytoskeleton Inc.) and active RhoA (BK124, Cytoskeleton Inc.) was performed in the same lysates. Scanning electron microscopy

(8) Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat DietFrontiers in pharmacologyJanuary 1, 2018Peng JH, Leng J, Tian HJ, Yang T, Fang Y, Feng Q, Zhao Y, Hu YY.G-LISA RhoA activation assay biochem kit (BK124, Cytoskeleton, Inc., CO, US), and total RhoA ELISA kit (BK150, Cytoskeleton, Inc., CO, US), respectively. The colon tissue sample was prepared according to the instruction

(9) Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: their emerging role in tumor heterogeneityScientific ReportsDecember 1, 2017Odessa Schillaci, Simona Fontana, Francesca Monteleone, Simona Taverna, Maria Antonietta Di Bella, Dolores Di Vizio, Riccardo Alessandrowas quantified by measuring the amounts of RhoA-GTP via the G-LISA RhoA Activation Biochem assay kit (BK150 Cytoskeleton, Inc, Denver, CO), an enzyme-linked immunosorbent assay (ELISA)-based. The total cellular

(10) Rho-associated kinase is a therapeutic target in neuroblastomaProceedings of the National Academy of SciencesAugust 8, 2017Cecilia Dyberg et al.Activity Assays. Total RhoA, active RhoA, and active Rac1 were investigated using ELISA and G-LISA kits (BK150, BK124, and BK128; Cytoskeleton). The RhoA G-LISA kit uses a plate coated with Rho-GTP– binding protein,

(11) Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDSNature immunologyAugust 1, 2016Park YH, Wood G, Kastner DL, Chae JJ.(BK121, cytoskeleton) and normalized to total RhoA levels, which were measured by Total RhoA ELISA kit (BK150, Cytoskeleton). RhoA-GTP levels of BMDMs treated with C3 toxin (0.5 μg/ml) and colchicine (102 – 104

(12) Rho kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closureJournal of Cell ScienceJuly 15, 2015Sarah Escuin, Bertrand Vernay, Dawn Savery, Christine B. Gurniak, Walter Witke, Nicholas D. E. Greene, Andrew J. Coppspectrophotometer reader (BioTek synergy HT). Total RhoA was determined by an ELISA assay (catalogue no. BK150, Cytoskeleton Inc.). Assays were performed with duplicate measures. Immunohistochemistry Gelatin was

(13) Tetraspanin CD9 Regulates Cell Contraction and Actin Arrangement via RhoA in Human Vascular Smooth Muscle CellsPLoS ONESeptember 3, 2014Michael J. Herr, Scott E. Mabry, Lisa K. JenningsGTP-bound RhoA Active and GTP-bound RhoA were measured from cell lysates using a total RhoA ELISA (BK150) or RhoA activation G-LISA kit (BK124) purchased from Cytoskeleton, Inc. (Denver, CO) per the manufacturer's

(14) The impact of flow-induced forces on the morphogenesis of the outflow tractFrontiers in PhysiologyJune 17, 2014Stefanie V. Biechler, Lorain Junor, Ashlie N. Evans, John F. Eberth, Robert L. Price, Jay D. Potts, Michael J. Yost, Richard L. Goodwinas described previously (Tan et al., 2012) using colorimetric ELISA assays (Total RhoA ELISA Kit, #BK150, Cytoskeleton Inc.; RhoA Activation Assay Biochem Kit, #BK124, Cytoskeleton Inc.). The percentage of

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RhoA ELISA Kit from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

RhoA ELISA Kit from Cytoskeleton, Inc.

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